HPLC and
analytical testing

Identity and purity testing for peptide materials, using high-performance liquid chromatography and liquid chromatography–mass spectrometry.

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Purity: HPLC
Identity: LC-MS
Reported: Per lot

Two questions sit behind every lot: is this the right compound, and how much of the sample is that compound? Analytical testing answers both.

Purity

How HPLC measures purity

High-performance liquid chromatography pushes a dissolved sample through a packed column under pressure. Different components travel through the column at different speeds, so they leave at different times and are detected separately.

The result is a chromatogram: a trace with a peak for each component. The main peak is the target compound, and its share of the total peak area is reported as purity.

Separates a sample into its components
Each peak represents a component
Purity is reported from the main peak’s share

Identity

What LC-MS adds

Purity alone does not confirm what a compound is. A sample could be pure and still be the wrong material.

Liquid chromatography–mass spectrometry adds a mass measurement to the separation. Comparing the measured mass with the expected mass supports confirmation of identity.

Certification

From data to Certificate of Analysis

Results are reported per lot and form the basis of the Certificate of Analysis. The certificate records the material, lot details, methods, results and test date.

A report relates to the lot tested. To check that a report applies to your material, compare the lot or batch ID printed on the unit with the one on the document.

Scope, equipment and accreditations for this service are to be confirmed before publication.

The process